Mammary cell line, AIB, transport, mammary tumor
Uptake of α-aminoisobutyric acid (AIB) by MCF-8, a line of malignant mouse mammary epithelial cells derived from a D2 hyperplastic nodule outgrowth, was found to be mediated by two different transport systems. In actively dividing cells about 60% of total AIB uptake is mediated by an A-like system: transport was Na+ -dependent, was inhibited by N-methyl-AIB, was maximal at pH 7.5 and ceased at pH 6. Transport by this system was reduced by nearly 65% when cell division ceased in confluent cultures, but the addition of insulin and/or calf serum to the medium of quiescent cells restored activity. Transport by the A system had a Km of 1.0 mM AIB and Vmax of 5.5 pM AIB transported per μg cellular protein per minute. About 20% of total AIB uptake was mediated by an L-like transport system which was Na+ -independent, was inhibited by α-2-aminobicyclo (2,2, l)-heptane-2-carboxylic acid, and was active at both pH 7.5 and pH 6. The activity of the L system was not influenced by cell growth rate or by insulin or serum. The remaining 20% of total AIB uptake occurs via a non-saturable route. No ASC-like system was detected.
Journal of the Iowa Academy of Science
© Copyright 1989 by the Iowa Academy of Science, Inc.
Voyles, Bruce A.
"Characterization of Aminoisobutyric Acid Transport Systems in MCF-8, a Line of Malignant Mouse Mammary Epithelial Cells,"
Journal of the Iowa Academy of Science: JIAS: Vol. 96:
, Article 9.
Available at: http://scholarworks.uni.edu/jias/vol96/iss1/9