fusion proteins, purification fusions, protein purification, ion-exchange, β-galactosidase
We explored the feasibility of attaching charged tails to a target protein, in this case β-galactosidase, for selective recovery. In this process, an ion-exchange membrane was used for selective binding and release of β-galactosidase with the attached purification fusions. Strength of binding and purity of eluate increased with increasing tail length. In addition, activity yield was improved with the implementation of an intermediate partial elution recycle procedure.
Journal of the Iowa Academy of Science
© Copyright 1994 by the Iowa Academy of Science, Inc.
Thiem, Erika A. and Heng, Meng H.
"Selective Recovery of β-Galactosidase With Charged Fusion Tails Using Ion-Exchange Membranes,"
The Journal of the Iowa Academy of Science: JIAS: Vol. 101:
, Article 10.
Available at: http://scholarworks.uni.edu/jias/vol101/iss2/10