Open Access Honors Program Thesis

First Advisor

David McClenahan


Adenosine triphosphate; Cattle--Diseases--Diagnosis; Pathogenic bacteria;


Extracellular ATP has been shown to increase the permeability of cells via the P2X7 receptor. This relationship could be important in understanding the physiology of the inflammation that occurs in bovine respiratory disease complex (BRDC). My research involved testing to see if this relationship between ATP and the P2X7 receptor is present. Then, potential inhibitors of ATP were tested so they can be used in further research involving ATP and BRDC. To test bovine cells, Mac-T cells were treated with ATP and incubated for various periods of time before Yo-Pro (a fluorescent molecule) was added. The percent change in fluorescence between cells with and without ATP determined the ability ATP had in opening the P2X7 receptor pore. Results showed that extracellular ATP does bind to the P2X7 receptor to open pores. In general, the greatest change in fluorescence was seen when ATP was incubated for at least 45 minutes before the Yo-Pro was added. The actual incubation time where the greatest change in fluorescence value occurred was 60 minutes. In addition, the results from the experiments with inhibitors A were insignificant; therefore no conclusions could be made at this time.

Date of Award



Department of Biology

University Honors Designation

A thesis submitted in partial fulfillment of the requirements for the designation University Honors

Date Original


Object Description

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