Complete Schedule

Title

Characterization of an Ancient Tetraploid Genome (Cypriniformes: Catostomidae)

Presentation Type

Poster Presentation

Keywords

Catostomidae--Genetics; Evolutionary genetics;

Abstract

Whole genome duplication (WGD) events result in the addition of thousands of duplicate genes and regulatory elements to the genome, which are subjected to a variety of evolutionary forces. Duplicates can experience a number of evolutionary fates including retention, loss of function by deleterious mutation resulting in pseudogenes, mutation resulting in novel function, or partitioning of function between copies. In the evolutionary history of animals, WGD events have occurred at several key points including a fish specific genome duplication event within Actinopterygii. Within the teleost fishes several additional groups have experienced further WGD events. Many of these have occurred within the order Cypriniformes including the family Catostomidae. The objective of this study is to examine the impact of WGD and subsequent genomic evolution on shaping the architecture of the tetraploid catostomid genome. Targeted enrichment techniques and high-throughput sequencing were used to selectively sequence the exome of eight species in the Catostomidae. Sequencing reads were de novo assembled. The exomes were characterized in reference to the well-annotated and closely related zebrafish genome by determining the number of single nucleotide changes, structural variations, and copy number variants. Patterns observed in these data were used to determine the amount of genome variation following WGD.

Start Date

4-4-2017 11:00 AM

End Date

4-4-2017 1:30 PM

Faculty Advisor

Peter Berendzen

Department

Department of Biology

Comments

Location: Maucker Union Ballroom

Embargo Date

4-4-2017

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Apr 4th, 11:00 AM Apr 4th, 1:30 PM

Characterization of an Ancient Tetraploid Genome (Cypriniformes: Catostomidae)

Whole genome duplication (WGD) events result in the addition of thousands of duplicate genes and regulatory elements to the genome, which are subjected to a variety of evolutionary forces. Duplicates can experience a number of evolutionary fates including retention, loss of function by deleterious mutation resulting in pseudogenes, mutation resulting in novel function, or partitioning of function between copies. In the evolutionary history of animals, WGD events have occurred at several key points including a fish specific genome duplication event within Actinopterygii. Within the teleost fishes several additional groups have experienced further WGD events. Many of these have occurred within the order Cypriniformes including the family Catostomidae. The objective of this study is to examine the impact of WGD and subsequent genomic evolution on shaping the architecture of the tetraploid catostomid genome. Targeted enrichment techniques and high-throughput sequencing were used to selectively sequence the exome of eight species in the Catostomidae. Sequencing reads were de novo assembled. The exomes were characterized in reference to the well-annotated and closely related zebrafish genome by determining the number of single nucleotide changes, structural variations, and copy number variants. Patterns observed in these data were used to determine the amount of genome variation following WGD.